The clinical application of self-inactivating (SIN) retroviral vectors has been hampered by the lack of reliable and efficient vector production technologies. To enable production of SIN γ-retroviral ...
Genomic DNA for Southern blot analysis was isolated from cells of the spleen or BM using the Blood DNA separation kit (Qiagen, Hilden, Germany). Genomic DNA (10 μg) was digested with appropriate ...
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